Effect of Density gradient technique on cryopreserved semen of infertile men using Glycyrrhiza glabra

Author:   Sura B.Sharafaldeen & Muhammed Abed Jawad

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Abstract

Eighty men, divided into five groups, (fifteen for each asthenozoospermic group, athenoteratozoospermic, asthenooligozoospermic, astheno-terato-oligospermic groups and twenty of normozoospermic group) were involved in this study. Ejaculated semen were analyzed and in vitro activation technique after cryopreservation-thawing was adopted by using Ham’s F-12 (control media) and Glycyrrhiza glabra (G.glabra)  to evaluate the sperm functional characteristics before and after cryopreservation and after activation of cryopreserved human semen.The results of all study groups showed a highly significant(P<0.001) improvement in certain sperm functional parameters compared with after thawing and before activation.                                                       

Keywords: G.glabra; asthenozoospermia